Evaluation Of Different Detections Methods Of Biofilm Formation In Urinary Isolates

Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. Urinary tract infection (UTI) is one of the leading causes of morbidity encountered in clinical practice. Biofilm produced by the urinary pathogens leads to recurrent and recalcitrant UTI there by contributing to longer stay in hospital and increased cost of treatment. The present study was conducted to evaluate Congo Red Agar method (CRA),TCP,MCRA and Tube Method (TM), in detection of biofilm formation in uropathogens with respect to Tissue Culture Plate method (TCP). A total of 2000 clinical isolates from urine samples will be subjected to different biofilm detection methods. All of the specimens will be collected from patients with nosocomial infections admitted to the L N. Hospital. the TCP method detected biofilm in 137 isolates (45.6%), TM detected biofilm in 118 isolates (39.3%), CRA and MCRA detected biofilm in 33 isolates (11%). The TCP was found to be most sensitive followed by the TM, CRA and the MCRA method. TCP method is the ideal method for detection of bacterial biofilm formation by uropathogens. MCRA method is superior to CRA and not to TCP or TM for detection of the staphylococcal biofilm formation.