Effect of CNP additive to diluents during capacitation on live percentage and pH of bull epididymal spermatozoa.
1Mohammedali J. Ghafil, Nazih W. Zaid, Najlaa S. Ibrahim, Hayder F. Nema
Abattoir male gonads specimens were collected from Al-Shoáalla abattoir from June 2019 until November 2019 in an average of 2 visits per week. This study was designed to evaluate the optimum effect of C-type natriuretic peptide on the caudal epididymal spermatozoa characteristics from a healthy slaughter bull. Dissected and separated the epididymis from the entire testicle made by a small scissor. The caudae were injected with 5-8 ml of the TCM199 media containing 100 IU/ml penicillin-streptomycin and 100 IU/ml Nystatin using an 18 gauge needle attached toa 5-ml syringe then aspirated. After collection of Spermatozoa samples of cauda epididymis were evaluated under the light microscope has individual motility less than 70% of were rejected. The caudal epididymal Spermatozoa samples transported from petri dish to a 10 ml test tube containing 2 ml maturation media (TCM199) that contain 100 IU/ml penicillin-streptomycin and 100 IU/ml Nystatin; the test tube incubated in a 5% CO2 incubator at 39 Cº for 4 hours for sperm maturation, the presence of distal protoplasmic droplet was the criteria of sperm maturation. Then the samples divided into three equal groups control one, low concentration 10-7 mol/L, and high concentration 10- 6 mol/L. It evaluated the effect of C-type natriuretic peptide (CNP) during capacitation on the live percentage and pH of bull epididymal spermatozoa. The live percentage and pH of sperm were analyzed at 5 min, 10 min, 15 min, 30 min, 45 min, 60 min, 120 min, and 180 min for each group. A total of 20 caudal epididymides were studied during the period of the experiment. Evaluation of spermatozoa parameters as a stained smear for dead and alive spermatozoa was conducted in addition to pH; all results were recorded.
Live percentage, pH, CNP, bull epidydims sperm.