Biological activity and phytochemical analysis of Acacia farnesiana extract against bacteria and antioxidant

1M. S. Al-Hamdani, Sundus Hammed, Saba Hadi, Saja Mohammed

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Abstract:

Objective: The interpretation and discussion of the conclusions of the scientific research depends mainly on the preliminary screening of phytochemicals of any plant known for its therapeutic effect of many common diseases. Therefore, the study was conducted to detect the essential compounds of acacia leaf extract Acacia farnesian leaves extract and their relationship to microbial growth, oxidation and its albumin inhibition. Methods: Phytochemical analysis of Acacia farnesianleaf extract was studied for the bioactive group's detection using standard methods, GC-Ms and FTI. Types of pathogenic bacteria as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiellaspp was used for antibacterial inhibition. Then the extract was undergo to antioxidant and anti-inflammatory examined using DPPH and for inhibition of albumin denaturation. Results: The results of detection of phytochemical of plant extract showed the high content of reduced sugars and flavonoids, then tannins, glycosides, Emodin, Comarine and protein at the modiest level, then the phenols and terpenoid at lowest level. The value of IC50forAcacia farnesian extract was 46.75μg/ml, while it was38.42 μg/mlfor ascorbic acid that used as control. It was found the albumin denaturation inhibition 80.9% close to 85.6% for Asprin as standard and have scavenging activity for free radical produced by DPPH . Conclusion: The study concluded with the high content of the secondary components of the Acacia farnesianplant, which has antioxidant properties and anti-albumin denaturation comparable to that of ascorbic acid.

Keywords:

Leaf extract, Secondary metabolites, GC-MS, FTIR, Anti-microbial

Paper Details
Month10
Year2020
Volume24
IssueIssue 10
Pages5527-5537