Comparison of 8-Hydroxy-Deoxyguanosine Levels in Cervical Cancer Advanced Stages Before and After Chemotherapy

1Sardina, Syahrul Rauf, Rina Previana Amiruddin, Siti Nur Asni, Maisuri Tadjuddin Chaliq, Sharvianty Arifuddin

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Abstract:

Cervical cancer is the most commonly diagnosed cancer and the third leading cause of death of women in poor and developing countries. 8-hydroxy-deoxyguanosine (8-OHdG) levels have been widely used as a biomarker of oxidative DNA damage including cervical cancer. This study aims to compare levels of 8- hydroxy-deoxyguanosine (8-OHdG) as a marker of oxidative stress in advanced cervical cancer (FIGO stages) before and after chemotherapy. This prospective study involved 18 patients stage IIB, 8 patients stage IIIA, 9 patients of stage IIIB and 2 patients stage IIIC of cervical cancer. 8-OHdG levels were measured with the ELISA method. The mean level of 8-OHdG before chemotherapy in stage II was 8.14±9.14 ng/ml and stage III 8.07±8.79 ng/ml whereas the mean level of 8-OHdG after chemotherapy in stage II was 24.24±12.46 ng/ml and at stage III 24.67±13.85 ng/ml. 8-OHdG levels increased significantly (p<.05) in stage IIA, IIIA and IIIB after chemotherapy. In contrast, 8-OHdG levels in stage IIIC was not significantly increased after chemotherapy. In addition, 8-OHdG levels were significantly different between SCC and adenocarcinoma. Likewise, the type of differentiation is good, moderate and non-classification. 8-OHdG levels increase significantly in advanced stages of cervical cancer after chemotherapy.

Keywords:

- 8-hydroxy-deoxyguanosine, cervical cancer, advanced stages

Paper Details
Month3
Year2020
Volume24
IssueIssue 3
Pages3971-3978