CORRELATION OF mRNA ALPHA RECEPTOR ESTROGEN (ER⍺) AND BETA RECEPTOR ESTROGEN (ERβ) EXPRESSION IN ADENOMYOSIS

1Rezky Juliana Putri Ridwan Gani, Nusratuddin Abdullah, Nurbani Bangsawan, Andi Alfian Zainuddin, Eddy Moeljono, Irnawati Bahar, Monika Fitria

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Abstract:

Adenomyosis is a condition in which endometrial tissue in the myometrium is hypertrophy and reactive. Adenomyosis is one of the gynecological problem that have an impact on a woman’s social life because very painful symptoms during menstruation and infertility. The exact pathogenesis of adenomyosis is still unclear. The estrogen is thought to be envolved in adenomyosis. Estrogen works on two estrogen receptors, ER⍺ and ERβ. Estrogen receptors thought to be an important step in the occurrence of adenomyosis. The exact mechanism regarding the role of ERα and Erβ is still being investigated. Research that analyzes the comparison of ERα and Erβ mRNA expression on the incidence of adenomyosis has never been done in Indonesia. This study aims to analyse the correlation of mRNA ERα and ERβ Expression in Adenomyosis. Research was done at educational hospital in Makassar from January to August 2019. an observational study with cross sectional study approach, a sample of 15 people diagnosed with Adenomyosis and 15 people diagnosed not Adenomyosis. Tissue were examine in the Pathology Anatomy Laboratory and Erα/Erβ mRNA expressions in Molecular Biology and Immunology Laboratory using Real Time PCR method. The Result of this study is ERα and ERβ mRNA expression increased in the adenomyosis group compared to the non-adenomyosis group. Pearson correlation showing strong positive correlation between ERα mRNA and ERβ mRNA expression with p-value of <0.0001 and correlation coefficient (r) of 0.692. In Adenomyosis, the expression of ERβ is higher than Erα, with an average Erβ mRNA expression value of 9.85 ±1.44, and mRNA Erα expression of 8.09 ±1.21. so there is a significant relationship between mRNA ERα and Erβ expression on the incidence of adenomyosis, especially more on Erβ.

Keywords:

Adenomyosis, ERα mRNA expression, Erβ mRNA expression

Paper Details
Month3
Year2020
Volume24
IssueIssue 3
Pages3953-3962