Genotypic Detection of rcsB, afaB, fim Hgenes Coding Biofilm Production of Escherichia Coli Isolated from Pressure Ulcer

1Taghreed Abdul Kareem Al-Makhzoomy

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Abstract:

Pressure ulcer or bed ulcer are localized injuries to the skin and or underlying tissue that commonlyhappen over a bony prominences, infected pressure ulcer pose a great risk when left untreated ,they can affected other body system .the most common bacterial type found in infected ulcer are Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus and Staphylococcus heamolyticus. Therefore 82 swab were collected from patient suffering from bed ulcer and admitted to Al-Sader medical city, and Al- Hakim General Hospitalthrough the term fromNovember 2018 to February 2019. The specimens was refined in suitable media(Mac Conky agar , and nutrient agar).after incubation period ,the result revealed that 119 bacteria isolates were isolated and identified according to vitek-2 system ,57(47.9%) of isolated were gram positive and 62(52.1%)were gram negative ,the gram negative represent by 25 isolated of Escherichia coli ,12 Klebsiella pneumonia ,14 Pseudomonas aeruginosa ,9 isolatesProutus mirabilis and one isolated for each Sertiaficania, Pseudomonas floursens. Genetically, study for rcsB, afaB, fim H gene encoding biofilm formation that associated with pathogensity of Escherichia coli by using PCR technique. The result of the present study reported that fimH gene was found in 23(92%) isolates of Escherichia coli, afaBgene was found in 19(76%) of Escherichia coli isolates and rcsB gene that 20(80%) of Escherichia coli isolates.

Keywords:

Escherichia Coli, rcsB, fimH, afabgenes.

Paper Details
Month4
Year2020
Volume24
IssueIssue 5
Pages5161-5167