Molecular Characterization of Waa Genes (waaA, waaC, wamB, wabG) Participate in Core Biosynthesis in P.mirabilis LPS

1S.H. Ruaa and Mayyada F. Darweesh

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Abstract:

LPS is a potential Proteus virulence factor that plays a key role in pathogenesis as well as in stimulating innate immune response. The structure of lipopolysaccharide differs from one bacterium to another, dependent on enzymes and gene products that can modify the basic structure of lipopolysaccharide in some bacteria, especially pathogens. So this study aimed to detection the present waa genes (waaA, waaC, wamB, wabG) that involve in biosynthesis of core LPS. 50 isolates of P. mirabilis were isolated from200 urine specimens taken from recurrent –urinary tract infections (UTI) patients attended to AL-Sadar Hospital. Specimens were cultured on specific media, and then bacterial isolates were identified depending on morphological, biochemical and VITK-2. The results showed that the. P. mirabilis comprise 11 (22%), 30(30%) and 9 (18 %) from recurrent UTI, kidney stone and Catheters samples respectively. The isolate that appeared multidrug resistance to all antibiotics used in this study were chosen to extracted lipopolysaccharide. Then this specimen was used for studies the genes responsible for synthesis of LPS,this specimen give positive result for waaA,waaC, wamB and wabG

Keywords:

P.mirabilis.LPS, Waagene, Molecular Characterization.

Paper Details
Month3
Year2020
Volume24
IssueIssue 5
Pages1453-1459