Molecular Detection of IntegronclassI Gene in Pseudomonas Aerginosa Isolated from Otitis Media

1Hala Ridha Abbas Al-Fahham

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Abstract:

The study aimed to investigating the Pseudomonas aerginosa from patients suffering otitis media infection. The study included of the bacterial diagnosis based on relied diagnostic procedures. A total of 40 specimens collected from (ear swab) in Al-Najaf province within period from September to December 2019.These patients ranged from 30-75 years. The male more than female. Primary identification was depended on Gram stain and biochemical tests. Finally identification with vitek 2 system was done, the results demonstrate the 30( 75%) to Pseudomonas aeruginosa. Genetically, study for gene encoding integrin of P. aeruginosa by using PCR technique. The results indicated, all P. aeruginosa isolate contain IntClassIgene that PCR amplification revealed that 30(75%) of P. aeruginosa carried int1gene.

Keywords:

P.aerginosa, IntclassI, Otitis Media.

Paper Details
Month3
Year2020
Volume24
IssueIssue 5
Pages1395-1398