Occurrence of Legionella spp. in Clinical and Environmental Samples in Babylon Province

1Abdulrasool Owayid Adday Azhar Althahab and Mourouge Saadi Alwash

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Abstract:

This study was designed to determine the occurrence of Legionella spp. recovered from clinical(sputum and dental wash) and environmental (hospital and domestic water system, air conditioner, showers and tap water)samples using standard biochemical testss. The all samples were diluted (1:10) in a KCl–HCl solution (pH 2.0), this was mixed and incubated at room temperature for 4s min, to avoid the growth of other undesired bacteria and to facilitate the better isolation of Legionella spp. Supernatant was discarded and the sediment was transferred and cultured on buffered buffered yeast extract (BCYE) agar culture medium. The culture media were incubated at 37 °C for36 hours without CO2 and 5 days in 2.5 % CO2. Degrees of the occurrence of Legionellapneumophilain clinical remains high (18.5%) in comparison with environmental samples (12.7%).

Keywords:

Babylon Province, Occurrence of Legionella, Clinical and Environmental

Paper Details
Month3
Year2020
Volume24
IssueIssue 5
Pages1369-1374