Optimization of Diagnostic Methods for Locally Advanced Forms of Cervical Cancer Based on Molecular Genetic Markers

1Nargiza Karimova, Doniyor Nishanov, Yashnar Mamadaliyeva

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Abstract:

Objective to improve the diagnosis of locally common forms of cervical cancer based on molecular genetic markers. Materials and methods: The study included 120 patients with locally advanced forms of cervical cancer (T2bN0-1M0, T3N0-1M0). In a comparative aspect, two groups of patients were studied. All patients received chemoradiotherapy: 3 courses of neo adjuvant chemotherapy were performed according to the cisplatin + 5 fluorouracil regimens, followed by a combination of radiation therapy, including remote gamma therapy, Sum. 46 Gy and intracavitary radiation therapy, ec Summary to point A 70-90 Gy, to point B 50-58 Gy. All biological materials obtained by biopsy were subjected to immunehistochemical studies. The immunohistochemical method was used to study Ki67, VEGF, p53 and the apoptosis regulator Вcl-2. The results of the analysis of the general three-year survival of patients with MRSHM, in which positive indicators of molecular genetic markers VEGF, Ki67, p53 were noted, according to the results of the study, it was revealed that of 21 patients who had high expression of the mutant p53 gene, after radical treatment, the overall three-year survival rate was 4.76%. The overall three-year survival of patients with positive VEGF protein figures was 13.6%, the expression of the Ki67 proliferative activity gene was 20.83%, and the overall and disease-free survival of patients with positive Bcl2 protein was statistically significant higher than the same values for cervical cancer (76.6%) (p<0.05). For example, with negative markers, the overall and relapse-fee three-year survival rates were high when, while maintaining a positive status, these indicators decreased (p <0.01).

Keywords:

VEGF, Ki67, Bcl2, p53, chemoradiotherapy, cervical cancer

Paper Details
Month2
Year2020
Volume24
IssueSpecial Issue 1
Pages691-700