Cytotoxicity Effect of Laserpitium Carduchorum Extract in Mcf-7 Human Breast Cancer Cells Via Cell Cycle Arrest and Induction if Apoptosis

1Dr. Twana Ahmed Mustafa, Hero Ismael Mohammed

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Abstract:

Laserpitium carduchorum is a perennial herb belonging to the Apiaceae family. In Iran, this plant is found in the Kurdistan Province, Bane Mountains. Several species of genus Laserpitium showed anti-bacterial, anti-fungal, cytotoxic, anti-tumor and anti-inflammatory activities. In this study, we investigated the effect of L. carduchorummethanolic extract on MCF-7 and HEK293 cell line by MTT assay, Induction of apoptosis, cell cycle arrest and expression of some apoptotic genes. After treatment of cells with plant extract, plant was significantly (P < 0.0001) cytotoxic to MCF-7 and cytotoxic to HEK293 at high dose treatment (1000μg/ml). IC50 for each cell evaluated. Proper IC50 for MCF-7 was 55.25μg/ml and 725.9μg/ml for HEK293. Cell cycle arrest and apoptosis were analyzed by using a FACSCanto II (BD Biosciences flow cytometry, cells arrested at S and G2/M phase(P<0.01, P<0.05)L. Carduchorum induced a marked increase (P < 0.001) in the percentage of early and late apoptotic. Real-time PCR was performed to analyze the expression of some apoptotic genes (Bax, Bcl-2, Caspase 3 and Caspase 9)Bax, a central cell death regulator, is an indispensable gateway to mitochondrial dysfunction and a major pro-apoptotic member of the B-cell lymphoma 2 was expressed significantly (p <0.05) after cells treatment of cell.

Keywords:

Laserpitium carduchorum, MCF-7, cell cycle, apoptosis.

Paper Details
Month2
Year2020
Volume24
IssueIssue 3
Pages1620-1628