Degradation Study Of Sofosbuvir In Bulk Drug And Tablet Dosage Form By RP-High Performance Liquid Chromatography

1Sushil D. Pati

2Mr. Kareemoddin A. Beldar

1MET Institute of Pharmacy, Bhujbal Knowledge City, Nashik, Savitribai Phule Pune University, Pune ,
2MET Institute of Pharmacy, Bhujbal Knowledge City, Nashik, Savitribai Phule Pune University, Pune

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Abstract:

Sofosbuvir were subjected to forced degradation conditions and the formed degradation products were well separated and resolved from the drug and excipients on SunFire C18 column (250 × 4.6 mm, 5 μ) using blend of Acetonitrile: Water (70: 30 v/v) pH 7.0 in isocratic mode at a flow rate of 0.8mL/min at an ambient temperature of 28 °C with the detection wavelength at 260nm. The retention time of Sofosbuvir was found 3.22 min. The linearity was performed in the concentration range of 20-100 ppm with correlation factor of 0.999 for Sofosbuvir. The percentage purity of Sofosbuvir tablet was found 99.98%. The drug was found to degrade under acid, alkali and oxidative condition but found stable under dry heat and photolytic conditions.

Keywords:

Sofosbuvir, RP-HPLC, Method Development, Method Validation, Stability.

Paper Details
Month11
Year2020
Volume24
IssueIssue 9
Pages45002-45008

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