Comparison of 8-Hydroxy-Deoxyguanosine Levels in Cervical Cancer Advanced Stages Before and After Chemotherapy

Cervical cancer is the most commonly diagnosed cancer and the third leading cause of death of women in poor and developing countries. 8-hydroxy-deoxyguanosine (8-OHdG) levels have been widely used as a biomarker of oxidative DNA damage including cervical cancer. This study aims to compare levels of 8- hydroxy-deoxyguanosine (8-OHdG) as a marker of oxidative stress in advanced cervical cancer (FIGO stages) before and after chemotherapy. This prospective study involved 18 patients stage IIB, 8 patients stage IIIA, 9 patients of stage IIIB and 2 patients stage IIIC of cervical cancer. 8-OHdG levels were measured with the ELISA method. The mean level of 8-OHdG before chemotherapy in stage II was 8.14±9.14 ng/ml and stage III 8.07±8.79 ng/ml whereas the mean level of 8-OHdG after chemotherapy in stage II was 24.24±12.46 ng/ml and at stage III 24.67±13.85 ng/ml. 8-OHdG levels increased significantly (p<.05) in stage IIA, IIIA and IIIB after chemotherapy. In contrast, 8-OHdG levels in stage IIIC was not significantly increased after chemotherapy. In addition, 8-OHdG levels were significantly different between SCC and adenocarcinoma. Likewise, the type of differentiation is good, moderate and non-classification. 8-OHdG levels increase significantly in advanced stages of cervical cancer after chemotherapy.