Degradation Study Of Sofosbuvir In Bulk Drug And Tablet Dosage Form By RP-High Performance Liquid Chromatography

Authors

  • Sushil D.Patil MET Institute of Pharmacy, Bhujbal Knowledge City, Nashik, Savitribai Phule Pune University, Pune Author
  • Mr.Kareemoddin A. Beldar MET Institute of Pharmacy, Bhujbal Knowledge City, Nashik, Savitribai Phule Pune University, Pune Author

DOI:

https://doi.org/10.61841/3tq7qp04

Keywords:

Sofosbuvir, RP-HPLC, Method Development, Method Validation, Stability

Abstract

Sofosbuvir was subjected to forced degradation conditions, and the formed degradation products were well separated and resolved from the drug and excipients on a SunFire C18 column (250 × 4.6 mm, 5 μ) using a blend of acetonitrile and water (70:30 v/v) at pH 7.0 in isocratic mode at a flow rate of 0.8 mL/min at an ambient temperature of 28 °C with the detection wavelength at 260nm. The retention time of Sofosbuvir was found to be 3.22 min. The linearity was performed in the concentration range of 20-100 ppm with a correlation factor of 0.999 for Sofosbuvir. The percentage purity of the Sofosbuvir tablet was found to be 99.98%. The drug was found to degrade under acid, alkali, and oxidative conditions but was found to be stable under dry heat and photolytic conditions. 

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References

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Published

30.11.2020

Issue

Vol. 24 No. 9 (2020): 24 (9) 2020

Section

Articles

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How to Cite

D.Patil, S., & A. Beldar, K. (2020). Degradation Study Of Sofosbuvir In Bulk Drug And Tablet Dosage Form By RP-High Performance Liquid Chromatography. International Journal of Psychosocial Rehabilitation, 24(9), 45002-45008. https://doi.org/10.61841/3tq7qp04