Results of experimental development of a laparoscopic method for auto transplantation of splenic tissue under a liver capsulе method
DOI:
https://doi.org/10.61841/c367wg62Keywords:
spleen, splenectomy, laparoscopy, liver, splenic autotransplantationAbstract
Despite the fact, spleen considers to be an important immunomodulating organ, splenectomy continues to be a primary tactic during extensive spleen injuries. Modern laparoscopic technologies force us to look for new low-invasive splenic autotransplantation methods. Meanwhile, the momentous aspect of such invasion is topographical and anatomical transplantation zone choosing, which allows maintaining the physiological connection between portal blood-flow and neo-splenic tissue. 47 experimental animals were included in exploration with the following steps of the study: experimental modeling laparoscopic splenic autotransplantation, dynamic clinical indicators monitoring of animals, experimental animal removal with further histological evaluation of neo-splenic tissue zone in the liver area. Clinical and histological data were evaluated on 7th, 15th, 30th and 60th days after surgery. Histological examination was provided by hematoxylin-eosin staining, magnification: eyepiece W-PL 10x / 23; A-Rlan 100x / 1.25 lens. Statistical data processing was performed using the program Statistica v.6.0. Clinical indicators normalization in the main group was observed by the 15th day. Absence of clinical blood parameters statistical difference between healthy animals and animals after surgery was observed by the 30th day (Р<0.05). The histological structure of the neo-splenic tissue and spleen of intact rabbits is identical, by the 60th day. Our experiment showed the feasibility of further splenic autotransplantation investigation and implementation of this method in endoscopic treatment of abdominal organs traumatic injuries.
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References
1. Alimov AN, et al. The choice of surgical treatment for spleen rupture with combined and isolated abdominal trauma from the position of an endosurgeon. Surgery. 2006; 3: 43-9.
2. Dynnik AG, Babich II. Modern representations and prospects for the development of splenic autotranslation in children Modern science: actual problems of theory and practice. Natural and Technical sciences. 2017; 2:55-60.
3. Morozov DA, Klyuev SA. Postsplenectomy hyposplenism. RAMS Herald. 2015; 70(4): 413-418.
4. Fayazov R. R., Mudarisova Z. G., Garipova Z. A. Prospects for organ-preserving and replacement technologies in spleen surgery. 2007; 2 (5): 25-9.
5. Acer SN, et al. Pediatric specific shock index accurately identifies severely injured children. J Pediatr Surg. 2015 53 (1): 130-135
6. Berger, M, et al. Laparoscopic-Assisted Versus Open Total Pancreatectomy and Islet Autotransplantation: A Case-Matched Study of Pediatric Patients. Journal of Pediatric Surgery, 2019; 0(0).
7. Cardoso, DL, et al. Should be considered splenic autotransplantation after total splenectomy due to trauma? Rev Col Bras Cir. 2018; 45 (3): 9
8. Coccolini F, et al. Splenic trauma. WSES classification and guidelines for adult and pediatric patients. World Journal of Emergency Surgery. 2017; 1:12-40.
9. Wu G, et al. Ex vivo resection of the giant epidermoid cyst and vascularized partial splenic autotransplantation: 3.5-year follow-up. Surgery. 2015; 158 (6): 1734-1737.
10. Haji S, et al. Spontaneous splenic rupture in a patient with light-chain deposition disease underlying autologous peripheral blood stem cell transplantation. The Japanese journal of clinical hematology. 2016; 57(6): 754-759.
11. Han B, et al. Regeneration of splenic autotransplants on liver attached by a tissue adhesive. Transplant Proc. 2010; 42 (5): 1944-1948
12. Xuan, et al. Management of intrahepatic splenosis: A case report and review of the literature. World Journal of Surgical Oncology. 2018; 16(1).
13. Linnaus M, et al. Prospective validation of the pediatric adjusted shock index (SIPA) in the blunt liver and spleen trauma. An ATOMAC study. Journal of pediatric surgery. 2017; 340-344.
14. Surendran A, et al. Splenic autotransplantation: a systematic review. ANZ Journal of Surgery. 2019;
15. Karahan O. et al. Evaluating the effectiveness of spleen autotransplantation into the liver and the omentum. MedicinePublished in Bratislavske lekarske listy. 2013; 130
16. Solovyeva E., Kondakov A. Decomposed piecewise polynomial predistortion for nonlinear power amplifier // 2015 International Siberian Conference on Control and Communications (SIBCON). Proceedings. – Omsk: Omsk State Technical University. Russia, Omsk, May 21−23, 2015, P.1-4. IEEE Catalog Number: CFP13794-CDR. ISBN: 978-1-4799-1060-1. DOI: 10.1109/SIBCON.2015.7147221
17. Solovyeva E. Operator approach to nonlinear compensator synthesis for communication systems // 2016 International Siberian Conference on Control and Communications (SIBCON). Proceedings. – Moscow: National Research University “Higher School of Economics”. Russia, Moscow, May 12−14, 2016, P.1–5. IEEE Catalog Number: CFP16794-CDR. Online ISSN: 2380-6516. DOI:
10.1109/SIBCON.2016.7491653
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