Gene expression and single nucleotide polymorphism (rs 1042838) of progesterone receptor gene in Iraqi females with recurrent spontaneous abortion(RSA)

The proposal target was determining the gene expression and single nucleotide polymorphism (rs1042838 G>T) of progesterone receptor gene in blood sample of recurrent spontaneous abortion RSA Iraqi females. Fifty females (n=50) with RSA consulted Al-Elwiya teaching hospital from February to June 2019 and apparently healthy fertile control (n=50) were enrolled. Following the serum hormonal assay, DNA and RNA were extracted from leucocytes to detriment the SNP and gene expression were carried out by (RT-q PCR). The results showed significant disruption of hormones between the two groups and non -significant difference (p>0.05) in the mean of threshold cycle(ΔCt) of progesterone receptor gene between female with RSA and control. Assessing the 2־ΔΔCt in female with RSA showed non -significant increased expression of folding in progesterone receptor m RNA compared with control. The genotypes and allele frequencies in the two groups showed significant decrease in the mutant (TT) genotype frequency in female with RSA. In conclusion, up-regulation of progesterone receptor gene expression was observed in female with RSA, the mutant genotype may play as protective SNP against RSA.