P16/Ki-67 Dual Staining Improve Detection of High-Grade Intraepithelial Lesions and Cervical Cancer

Worldwide, cervical intraepithelial lesions are common in adult women , after the age of 30 years . The high grade lesions are precancerous and could transformed to in situ and invasive squamous cell carcinoma of uterian cervix .These transformation significantly can be reduced by screening program of the cervix ,namely : cytology, HR-HPV test , and colposcopy . Women with cytology of utrian cervix that showed morphology of intraepithelial lesions ,especially high grade lesions , HR-HPV test should be done for these women . However , high prevalence of HPV infection can`t predicate the risk of malignant transformation in these women , thus additional triage test , including biomarkers for transformed cells can be used to detect women need further evaluation , follow up and treatment . In current study , p16/Ki-67 dual immunostaining , was used for cytology as biomarker for detection of cellular changes and can improve the diagnosis of precancerous lesions and cervical squamous cells carcinoma