The Protective Role of Rutin on some Biochemical and histological parameters induced-hyperlipidemia by Triton WR1339 in male rats
DOI:
https://doi.org/10.61841/z3z26325Keywords:
Rutin, hyperlipidemia, Triton WR1339, male ratsAbstract
Rutin (3,3, 4′, 5, 7-pentahydrohyflavone-3-rhamnoglucoside) is a flavonoid of the flavonol type. Rutin is found in many plants and is also an important dietary constituent of food and plant-based beverages. Rutin has several pharmacological properties including antioxidant activities. Also, it was identified that rutin is the major low-density lipoprotein (LDL) antioxidant compound of mulberry in an study. The effects of rutin were tested by using it as induced-hyperlipidemia by Triton WR1339. Male rats were fed 4 weeks with rutin (30mg/kg) to study the hyperlipidemia effects of rutin on serum lipid levels, hepatic enzyme activity, and liver tissue. gavaged the animals with Triton WR1339 resulted in marked hyperlipidemia and increased the serum level of LDL cholesterol (LDL-C). Rutin (at 30 mg/kg) alone significantly reduced the levels of total cholesterol, and LDL-C and also markedly decreased liver enzymes and weight in animals with a high-cholesterol diet. Our findings show that 30 mg/kg of rutin alone or with other supplementation lowered liver weight and enzymes as well as serum total cholesterol and LDL. The hepatic histopathological results reflect the correlation of rutin with liver weight and the levels of serum total cholesterol and LDL-C. These results indicate that rutin has increased anti hyperlipidemia effects in an animal model.
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1. Loscalzo J, Libby P, Braunwald E. Cardiovascular disorders. In: Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL, Jameson JL, editors. Harrison’s Principles of Internal Medicine. New York: McGraw-Hill; 2008. p. 1375–1505.
2. Byington RP, Jukema JW, Salonen JT, Pitt B, Bruschke AV, Hoen H, et al. Reduction in cardiovascular events during pravastatin therapy: pooled analysis of clinical events of the pravastatin atherosclerosis intervention program. Circulation. 1995;92:2419–2425.
3. Delsing DJ, Offerman EH, van Duyvenvoorde W, van der Boom H, de Wit EC, Gijbels MJ, et al. Acyl-CoA: cholesterol acyltransferase inhibitor avasimibe reduces atherosclerosis in addition to its cholesterol-lowering effect in ApoE*3-Leiden mice. Circulation. 2001;103:1778–1786.
4. Nofer JR, Kehrel B, Fobker M, Levkau B, Assmann G, von Eckardstein A. HDL and arteriosclerosis: beyond reverse cholesterol transport. Atherosclerosis. 2002;161:1–16.
5. Harnafi H, Caid HS, Bouanani NH, Aziz M, Amrani S. Hypolipemic activity of polyphenol-rich extracts from Ocimum basilicum in Triton WR-1339-induced hyperlipidemic mice. Food Chemistry. 2008;108:205–212.
6. Bertges LC, Mourão CA Jr, Souza JB, Cardoso VAC. Hyperlipidemia induced by Triton WR-1339 (Tyloxapol) in Wistar rats. Revista Brasileira de Ciências Médicas e Saúde. 2011;1(1):32–34.
7. Janicki BS, Aron SA. Effect of Triton WR-1339 on lipoproteins and lipoprotein lipase of guinea pig serum. Proceedings of the Society for Experimental Biology and Medicine. 1962;109:507–509.
8. Bok SH, Lee SH, Park YB, Bae KH, Son KH, Jeong TS. Plasma and hepatic cholesterol and hepatic activities of HMG-CoA reductase and acyl-CoA cholesterol acyltransferase in rats fed citrus peel extract. Journal of Nutrition. 1999;129:1182–1185.
9. Havsteen BH. The biochemistry and medical significance of flavonoids. Pharmacology & Therapeutics. 2002;96:67–202.
10. Arai Y, Watanabe S, Kimira M, Shimoi K, Mochizuki R, Kinae N. Dietary intakes of flavonols, flavones, and isoflavones by Japanese women. Journal of Nutrition. 2000;130:2243–2250.
11. Kuntic V, Pejic N, Ivkovic B, Vujic Z, Ilic K, Micic S. Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. Journal of Pharmaceutical and Biomedical Analysis. 2007;43:718–721.
12. Schwedhelm E, Maas R, Troost R, Böger RH. Clinical pharmacokinetics of antioxidants and their impact on systemic oxidative stress. Clinical Pharmacokinetics. 2003;42:437–459.
13. Sheu JR, Hsiao G, Chou PH, Shen MY, Chou DS. Mechanisms involved in the antiplatelet activity of rutin in human platelets. Journal of Agricultural and Food Chemistry. 2004;52:4414–4418.
14. Janbaz KH, Saeed SA, Gilani AH. Protective effect of rutin on paracetamol- and CCl₄-induced hepatotoxicity in rodents. Fitoterapia. 2002;73:557–563.
15. La Casa C, Villegas I, Alarcón de la Lastra C, Motilva V, Martín Calero MJ. Protective and antioxidant properties of rutin against ethanol-induced gastric lesions. Journal of Ethnopharmacology. 2000;71:45–53.
16. Mellou F, Loutrari H, Stamatis H, Roussos C, Kolisis FN. Enzymatic esterification of flavonoids with unsaturated fatty acids. Process Biochemistry. 2006;41:2029–2034.
17. Trumbeckaite S, Bernatoniene J, Majiene D, Jakstas V, Savickas A, Toleikis A. Effect of flavonoids on rat heart mitochondrial function. Biomedicine & Pharmacotherapy. 2006;60:245–248.
18. Nassiri-Asl M, Shariati-Rad S, Zamansoltani F. Anticonvulsive effects of intracerebroventricular administration of rutin in rats. Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2008;32:989–993.
19. Lebeau J, Neviere R, Cotelle N. Beneficial effects of flavonoids after ischemia-reperfusion in isolated rat heart. Bioorganic & Medicinal Chemistry Letters. 2001;11:23–27.
20. De Celle T, Heeringa P, Strzelecka AE, Bast A, Smits JF, Janssen BJ. Protective effects of 7-monohydroxyethylrutoside in cardiac ischemia-reperfusion. European Journal of Pharmacology. 2004;491:205–212.
21. Gupta R, Singh M, Sharma A. Neuroprotective effect of antioxidants in cerebral ischemia-reperfusion injury. Pharmacological Research. 2003;48:209–215.
22. Neumayer C, Fugl A, Nanobashvili J, Blumer R, Punz A, Gruber H, et al. Combined enzymatic and antioxidative treatment reduces ischemia-reperfusion injury. Journal of Surgical Research. 2006;133:150–158.
23. Katsube T, Imawaka N, Kawano Y, Yamazaki Y, Shiwaku K, Yamane Y. Antioxidant flavonol glycosides in mulberry leaves. Food Chemistry. 2006;97:25–31.
24. Vogel HG, Vogel WH. Drug Discovery and Evaluation: Pharmacological Assays. Berlin: Springer; 1997.
25. Jeon SM, Park YB, Choi MS. Antihypercholesterolemic property of naringin in rabbits. Clinical Nutrition. 2004;23:1025–1034.
26. Moghadasian MH. Experimental atherosclerosis: a historical overview. Life Sciences. 2002;70:855–865.
27. Burstein PJ, Draznin B, Johnson CJ, Schalch DS. Effect of hypothyroidism on growth hormone and IGF in rats. Endocrinology. 1979;104:1107–1111.
28. Choe S, Kim H, Jeong T, Bok S, Park Y. Naringin inhibits adhesion molecules in hypercholesterolemic rabbits. Cardiovascular Pharmacology. 2001;38:947–955.
29. Kanhal MA, Ahmad F, Othman AA, Arif Z, Orf S, Murshed KS. Effects of cholesterol-rich diets on biochemical parameters in rats. International Journal of Food and Nutrition. 2002;53:381–388.
30. Borradaile NM, Carroll KK, Kurowska EM. Regulation of apolipoprotein B metabolism by citrus flavanones. Lipids. 1999;34:591–598.
31. Attaway JA, Buslig BS. Antithrombogenic and antiatherogenic effects of citrus flavonoids. Advances in Experimental Medicine and Biology. 1998;439:165–173.
32. Cervantes-Laurean D, Schramm DD, Jacobson EL, Halaweish I, Bruckner GG, Boissonneault GA. Inhibition of AGE formation by rutin. Journal of Nutritional Biochemistry. 2006;17:531–540.
33. Bok SH, Park SY, Park YB, Lee MK, Jeon SM, Seong TS. Effects of quercetin on lipid metabolism. International Journal for Vitamin and Nutrition Research. 2002;72:161–169.
34. Becker EM, Ntouma G, Skibsted LH. Synergism and antagonism between quercetin and antioxidants. Food Chemistry. 2007;103:1288–1296.
35. Middleton E Jr, Kandaswami C, Theoharides TC. Effects of plant flavonoids on mammalian cells. Pharmacological Reviews. 2000;52:673–751.
36. Lopez-Revuelta A, Sanchez-Gallego JA, Hernandez-Hernandez A, Sanchez-Yague J, Llanillo M. Protective effects of quercetin and rutin in erythrocytes. Chemico-Biological Interactions. 2006;161:79–91.
37. Kim H, Kong H, Choi B, Yang Y, Kim Y, Lim MJ. Pharmacological properties of rutin in inflammatory bowel disease. Pharmaceutical Research. 2005;22:1499–1509.
38. Kwon KH, Murakami A, Tanaka T, Ohigashi H. Dietary rutin ameliorates experimental colitis. Biochemical Pharmacology. 2005;69:395–406.
39. Rotelli AE, Guardia T, Juárez AO, de la Rocha NE, Pelzer LE. Comparative study of flavonoids in inflammation models. Pharmacological Research. 2003;48:601–606.
40. Farmer JA. Aggressive lipid therapy in the statin era. Progress in Cardiovascular Diseases. 1998;41:71–94.
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