Isolating and Identification germs of Staphylococcus aureus from of butchers' Shops Instrument in the Shekhan District
DOI:
https://doi.org/10.61841/asyrs569Keywords:
Instruments Butchers, Bacteria, Staphylococcus aureus, Sheikhan distractAbstract
The aim of the study was conducted to isolation microbial contamination in butchers instruments and meat in shekhan district, and identify the most important bacterial species associated with these instruments. 65 samples were taking from different localities of Shekhan district distributed among (9 samples from kalakchi area, 15 samples from chera area, 21 samples from qasrok area and 20 samples from shekhan) After taken sample by a swab from tools of butcher's shops (Knife ,hooks , cutting board and meat grinder) after that the samples were taken to the laboratory of shekhan technical colleague of health for completion process of culturing, isolated and examined of the bacterial species and studying the morphological biochemical characteristics of bacteria by using culture media. The results of the current study showed growth six different species of bacteria were Staphylococcus aureus 24 samples (36%) Staphylococcus epidermidis 9 samples (14%), Staphylococcus saprophytes 5 samples (8%), Streptococcus 9 samples (14%), Bacilli species 2 sample (3%) , Gram negative species 16 samples (25%) . At the conclusion of this study, two types of bacteria were isolated the first of which was gram negative and its proportion was (25%) the second was gram positive and its proportion was (75%), Identified six species of bacteria was staphylococcus aureus, staphylococcus epidermidis, staphylococcus saprophytous, streptococcus and bacilli species.
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References
1. Baron, S. (1996). Alphaviruses (Togaviridae) and Flaviviruses (Flaviviridae). In Medical Microbiology. University of Texas Medical Branch at Galveston.
2. Boler, D.D., & Woerner, D.R. (2017). What is meat? A perspective from the American Meat Science Association. Animal Frontiers, 7(4), 8–11.
3. Bouvet, C., Gjoni, S., Zenelaj, B., Lipsky, B.A., Hakko, E., & Uçkay, I. (2017). Staphylococcus aureus soft tissue infection may increase the risk of subsequent staphylococcal soft tissue infections. International Journal of Infectious Diseases, 60, 44–48.
4. Canencia, O.P., Dalugdug, M.D., Emano, A.M.B., Mendoza, R.C., & Walag, A.M.P. (2016). Slaughter waste effluents and river catchment watershed contamination in Cagayan de Oro City, Philippines. J Biodivers Conserv Environ Sci, 9(2), 142–148.
5. Cohen-Poradosu, R., & Dennis, L. (2007). Group A Streptococcus Epidemiology and Vaccine Implications. Clinical Infectious Diseases, 45(7), 863–865. https://doi.org/10.1086/521263
6. Ferretti, J.J., et al. (2001). Complete genome sequence of an M1 strain of Streptococcus pyogenes. PNAS, 98(8), 4658–4663. https://doi.org/10.1073/pnas.071559398
7. Fong, F. (2017). Food Safety Platform: Bacteria in Raw Meat vs Cooked Meat. Food Safety Focus, 130 (May 2017).
8. Fredrickson, J.K., et al. (2004). Geomicrobiology of high-level nuclear waste contaminated vadose sediments at the Hanford Site, Washington State. Applied and Environmental Microbiology, 70(7), 4230–4241.
9. CDC (2007). Group A Streptococcal (GAS) Disease. Centers for Disease Control and Prevention. (Archived December 19). Retrieved November 21, 2012.
10. Kuroda, M., et al. (2005). Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection. PNAS, 102(37), 13272–13277.
11. Lawrie, R.A., & Ledward, D.A. (2006). Lawrie's Meat Science (7th ed.). Cambridge: Woodhead Publishing Limited.
12. Masalha, M., et al. (2001). Analysis of transcription of the Staphylococcus aureus aerobic class Ib and anaerobic class III ribonucleotide reductase genes in response to oxygen. Journal of Bacteriology, 183(24), 7260–7272.
13. O’Gara, J.P., & Humphreys, H. (2001). Staphylococcus epidermidis biofilms: importance and implications. Journal of Medical Microbiology, 50(7), 582–587.
14. Oniciuc, E.A., Nicolau, A.I., Hernández, M., & Rodríguez-Lázaro, D. (2017). Presence of methicillin-resistant Staphylococcus aureus in the food chain. Trends in Food Science & Technology, 61, 49–59.
15. Rappé, M.S., & Giovannoni, S.J. (2003). The uncultured microbial majority. Annual Review of Microbiology, 57, 369–394.
16. Schleifer, K.H., & Kloos, W.E. (1975). Isolation and characterization of staphylococci from human skin. International Journal of Systematic and Evolutionary Microbiology, 25(1), 50–61.
17. Tong, S.Y., Davis, J.S., Eichenberger, E., Holland, T.L., & Fowler, V.G. (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical Microbiology Reviews, 28(3), 603–661.
18. Tuazon, C.U., et al. (1979). Serious infections from Bacillus sp. JAMA, 241(11), 1137–1140.
19. Zhang, X., Owens, C.M., & Schilling, M.W. (2017). Meat: the edible flesh from mammals only or does it include poultry, fish, and seafood? Animal Frontiers, 7(4), 12–18.
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