Optimization of production of phycobiliproteins from Spirulina maxima and Spirulina platensis under laboratory conditions
DOI:
https://doi.org/10.61841/3g4msx40Keywords:
Spirulina platensis, Spirulina maxima, phycobiliproteins, phycocyanin, phycoerythrin, allophycocyaninAbstract
Cyanobacteria, especially Spirulina sp., are considered as valuable tool for obtaining phycobiliproteins, which has numerous applications. The present study was conducted to optimize the production of phycobiliproteins from two strains of Spirulina, i.e. S. maxima and S. platensis, grown in CFTRI medium. The optimization was done by changing the light colour, nitrate source and pH of the medium. The results showed that monochromatic light, in red zone, nitrate source and sodium and potassium nitrate as well as pH 10.0 enhanced the production of three major components of phycobiliproteins, phycocyanin (PC), phycoerythrin (PE) and allophycocyanin (APC), as well as chlorophyll a concentration and total cellular proteins, after 30 days of growth. Further, S. platensis proved to be better strain as compared to S. maxima for production of phycobiliproteins in CFTRI medium.
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1. Garlapati, D., Chandrasekaran, M., Devanesan, A., Mathimani, T. and Pugazhendhi, A., 2019. Role of cyanobacteria in agricultural and industrial sectors: an outlook on economically important byproducts. Applied microbiology and biotechnology, 103(12), pp.4709-4721.
2. Singh, N.K., Parmar, A. and Madamwar, D., 2009. Optimization of medium components for increased production of C-phycocyanin from Phormidium ceylanicum and its purification by single step process. Bioresource technology, 100(4), pp.1663-1669.
3. Saini, D.K., Pabbi, S. and Shukla, P., 2018. Cyanobacterial pigments: perspectives and biotechnological approaches. Food and chemical toxicology, 120, pp.616-624.
4. Rodríguez-Sánchez, R., Ortiz-Butrón, R., Blas-Valdivia, V., Hernández-García, A. and Cano-Europa, E., 2012. Phycobiliproteins or C-phycocyanin of Arthrospira (Spirulina) maxima protect against HgCl2-caused oxidative stress and renal damage. Food chemistry, 135(4), pp.2359-2365.
5. Jaiswal, A., Koli, D.K., Kumar, A., Kumar, S. and Sagar, S., 2018. Pigments analysis of cyanobacterial strains. IJCS, 6(2), pp.1248-1251.
6. Fatma, T., 2009. Screening of cyanobacteria for phycobiliproteins and effect of different environmental stress on its yield. Bulletin of environmental contamination and toxicology, 83(4), p.509.
7. Mandal, M.K., Chanu, N.K. and Chaurasia, N., 2020. Cyanobacterial pigments and their fluorescence characteristics: applications in research and industry. In Advances in Cyanobacterial Biology (pp. 55-72). Academic Press.
8. Ajayan, K.V., Selvaraju, M. and Thirugnanamoorthy, K., 2012. Enrichment of chlorophyll and phycobiliproteins in Spirulina platensis by the use of reflector light and nitrogen sources: An in-vitro study. Biomass and bioenergy, 47, pp.436-441.
9. Muramatsu, M. and Hihara, Y., 2012. Acclimation to high-light conditions in cyanobacteria: from gene expression to physiological responses. Journal of plant research, 125(1), pp.11-39.
10. Singh, N.K., Parmar, A., Sonani, R.R. and Madamwar, D., 2012. Isolation, identification and characterization of novel thermotolerant Oscillatoria sp. N9DM: change in pigmentation profile in response to temperature. Process biochemistry, 47(12), pp.2472-2479.
11. Salunke, K.J., Magar, S.A., Joshi, R.R. and Wadikar, M.S., 2016. Comparative study on growth of Spirulina platensis on different culture media. Biosci. Discov., 7(1), pp.90-92.
12. Thingujam, I., Keithellakpam, O.S., Oinam, A.S., Oinam, G., Nato, T.O. and Dutt, S.G., 2016. Optimization of chlorophyll a production of some cyanobacteria from rice paddies in Manipur, India, through nutritional and environmental factors. Philippine J Sci, 145(4), pp.373-383.
13. Nath, A., Vajpayee, G., Dixit, K., Rahman, A., Kannaujiya, V.K. and Sundaram, S., 2017. Micro-algal consortia complexity enhances ecological biomass stability through CO2 sequestration. J Algal Biomass Utln, 8, pp.19-34.
14. Bennett, A. and Bogorad, L., 1973. Complementary chromatic adaptation in a filamentous blue-green alga. The Journal of cell biology, 58(2), pp.419-435.
15. Johnson, E.M., Kumar, K. and Das, D., 2014. Physicochemical parameters optimization, and purification of phycobiliproteins from the isolated Nostoc sp. Bioresource technology, 166, pp.541-547.
16. Sekar, S. and Chandramohan, M., 2008. Phycobiliproteins as a commodity: trends in applied research, patents and commercialization. Journal of Applied Phycology, 20(2), pp.113-136.
17. Garlapati, D., Chandrasekaran, M., Devanesan, A., Mathimani, T. and Pugazhendhi, A., 2019. Role of cyanobacteria in agricultural and industrial sectors: an outlook on economically important byproducts. Applied microbiology and biotechnology, 103(12), pp.4709-4721.
18. Zeng, X., Danquah, M.K., Zhang, S., Zhang, X., Wu, M., Chen, X.D., Ng, I.S., Jing, K. and Lu, Y., 2012. Autotrophic cultivation of Spirulina platensis for CO2 fixation and phycocyanin production. Chemical Engineering Journal, 183, pp.192-197.
19. Sala, L., Ores, J.D.C., Moraes, C.C. and Kalil, S.J., 2018. Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52. The Canadian Journal of Chemical Engineering, 96(9), pp.1896-1902.
20. Castro-García, S.Z., Chamorro-Cevallos, G., Quevedo-Corona, L., McCarty, M.F. and Bobadilla-Lugo, R.A., 2018. Beneficial effects of phycobiliproteins from Spirulina maxima in a preeclampsia model. Life sciences, 211, pp.17-24.
21. Soundarapandian, P. and Vasanthi, B., 2008. Effects of chemical parameters on Spirulina platensis biomass production: Optimized method for phycocyanin extraction. Int J Zool Res, 4(1), pp.1-11.
22. Kenekar, A.A. and Deodhar, M.A., 2014. Operational strategies for lab scale horizontal tubular photobioreactor for mitigation of CO2 using an indigenous thermophilic microalgal strain Geitlerinema sulphureum. Journal of Petroleum and Environmental Biotechnology, 5(3), pp.1-8.
23. Khazi, M., Demirel, Z. and Conk, D.M., 2018. Enhancement of biomass and phycocyanin content of Spirulina platensis. Front Biosci, 10, pp.276-286.
24. Kilimtzidi, E., Cuellar Bermudez, S., Markou, G., Goiris, K., Vandamme, D. and Muylaert, K., 2019. Enhanced phycocyanin and protein content of Arthrospira by applying neutral density and red light shading filters: a small‐scale pilot experiment. Journal of Chemical Technology & Biotechnology, 94(6), pp.2047- 2054.
25. Gupta, A., Mohan, D., Saxena, R.K. and Singh, S., 2018. Phototrophic cultivation of NaCl‐tolerant mutant of Spirulina platensis for enhanced C‐phycocyanin production under optimized culture conditions and its dynamic modeling. Journal of phycology, 54(1), pp.44-55.
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