Correlation of interleukin-6 gene expression and idiopathic female infertility

Authors

  • Shaymaa Rabeea Madhkhoor College of Biotechnology, University of Al-Qadisiyah, Iraq Author
  • Jameel K. Alumeri Department of Biology, College of Education, University of Al-Qadisiyah, Iraq. Author

DOI:

https://doi.org/10.61841/tc7m7p27

Keywords:

ELISA, female, infertility, interleukin, RT-qPCR.

Abstract

The present investigation was focused on the identification of the interleukin-6 (IL-6) role in the unexplained female infertility. According to this aim, 100 blood samples were collected from fertile (T1), 50 samples, and infertile (T2), 50 samples, women who attended Al-Nasiriyah Children and Obstetrics Hospital, Al-Nasiriyah, Iraq. Enzyme-linked immunosorbent assay (ELISA) detection was performed to recognize the IL-6 concentrations in the blood samples of those women. A real time-quantitative polymerase chain reaction (RT-qPCR) method was used to identify the gene expression (GE) of the IL-6 gene in the blood of those women. The results of the ELISA showed significant (p˂0.05) higher concentrations of the IL-6 in the blood samples of the T2 women, 352.25pg/ml, than those in the blood samples of the T1 women, 152.35pg/ml. Moreover, the RT-qPCR revealed significant (p˂0.05) higher relative GE of the IL-6 gene in the blood samples of the T2 women, 6.892-fold change (FC), than that in the blood samples of the T1 women, 2.488 FC. The current study indicates that IL-6 may have a major role in the unexplained female infertility.

Downloads

Download data is not yet available.

References

1. Ahn, S. H. et al. (2015) ‘IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors’, The Journal of Immunology, 195(6), pp. 2591– 2600. doi: 10.4049/jimmunol.1501138.

2. An, L.-F. et al. (2015) ‘Unexplained infertility patients have increased serum IL-2, IL-4, IL-6, IL-8, IL-21, TNFα, IFNγ and increased Tfh/CD4 T cell ratio: increased Tfh and IL-21 strongly correlate with presence of autoantibodies’, Immunological Investigations, 44(2), pp. 164–173. doi: 10.3109/08820139.2014.932377.

3. Demir, B. et al. (2009) ‘ORIGINAL ARTICLE: Serum IL-6 Level May Have Role in the Pathophysiology of Unexplained Infertility’, American Journal of Reproductive Immunology, 62(4), pp. 261–267. doi: 10.1111/j.1600-0897.2009.00734.x.

4. HATASAKA, H. (2011) ‘New Perspectives for Unexplained Infertility’, Clinical Obstetrics and Gynecology, 54(4), pp. 727–733. doi: 10.1097/GRF.0b013e3182353e54.

5. Isaksson, R. and Tiitinen, A. (2004) ‘Present concept of unexplained infertility.’, Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, 18(5), pp. 278–90. Available at: http://www.ncbi.nlm.nih.gov/pubmed/15346664 (Accessed: 6 April 2019).

6. Lessey, B. A. and Kim, J. J. (2017) ‘Endometrial receptivity in the eutopic endometrium of women with endometriosis: it is affected, and let me show you why.’, Fertility and sterility. NIH Public Access, 108(1),

pp. 19–27. doi: 10.1016/j.fertnstert.2017.05.031.

7. Malcolm, C. E. and Cumming, D. C. (2003) ‘Does anovulation exist in eumenorrheic women?’, Obstetrics and gynecology, 102(2), pp. 317–8. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12907106 (Accessed: 6 April 2019).

8. McGovern;, A. M. (2017) ‘Unexplained Infertility’, Topics in Obstetrics &amp, 37(5), pp. 1–5. doi: 10.1097/01.pgo.0000513371.76520.9f.

9. Practice Committee of the American Society for Reproductive Medicine (2006a) ‘Effectiveness and treatment for unexplained infertility’, Fertility and Sterility, 86(5), pp. S111–S114. doi: 10.1016/j.fertnstert.2006.07.1475.

10. Practice Committee of the American Society for Reproductive Medicine (2006b) ‘Optimal evaluation of the infertile female’, Fertility and Sterility, 86(5), pp. S264–S267. doi: 10.1016/j.fertnstert.2006.08.041.

11. Quaas, A. and Dokras, A. (2008) ‘Diagnosis and treatment of unexplained infertility.’, Reviews in obstetrics & gynecology. MedReviews, LLC, 1(2), pp. 69–76. Available at: http://www.ncbi.nlm.nih.gov/pubmed/18769664 (Accessed: 6 April 2019).

12. Mima, T., Nishimoto, N., (2009). Clinical value of blocking IL-6 receptor. Curr. Opin. Rheumatol. 21, 224–230.

13. Sikorski, K., Czerwoniec, A., Bujnicki, J.M., Wesoly, J., Bluyssen, H.A., (2011). STAT1 as a novel therapeutical target in pro-atherogenic signal integration of IFNgamma TLR4 and IL-6 in vascular disease. Cytokine Growth Factor Rev. 22, 211–219.

14. Clark, D.A., Coulam, C.B., Daya, S., Chaouat, G., (2001). Unexplained sporadic and recurrent miscarrage in the new millennium: a critical analysis of immune mechanisms and treatments. Hum. Reprod. Update 7, 501–511.

15. Guerin, L.R., Prins, J.R., Robertson, S.A., (2009). Regulatory T-cells and immune tolerance in pregnancy: a new target for infertility treatment? Hum. Reprod. Update 15, 517–535.

16. Demir, B., Guven, S., Guven, E.S., Atamer, Y., Gul, T., (2009). Serum IL-6 level may have role in the pathophysiology of unexplained infertility. Am. J. Reprod. Immunol. 62, 261–267.

17. Sherwin, J.R., Smith, S.K., Wilson, A., Sharkey, A.M., (2002). Soluble gp130 is up-regulated in the implantation window and shows altered secretion in patients with primary unexplained infertility. J. Clin. Endocrinol. Metab. 87, 3953–3960.

18. Jasper, M.J., Tremellen, K.P., Robertson, S.A., (2006). Primary unexplained infertility is associated with reduced expression of the T-regulatory cell transcription factor Foxp3 in endometrial tissue. Mol. Hum. Reprod. 12, 301–308.

Downloads

Published

30.06.2020

Issue

Vol. 24 No. 6 (2020): 24 (6) 2020

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

You are free to:

  1. Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
  2. Adapt — remix, transform, and build upon the material for any purpose, even commercially.
  3. The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

  1. Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  2. No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Notices:

You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .

No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.

How to Cite

Madhkhoor, S. R., & Alumeri, J. K. (2020). Correlation of interleukin-6 gene expression and idiopathic female infertility. International Journal of Psychosocial Rehabilitation, 24(6), 11975-11980. https://doi.org/10.61841/tc7m7p27