Evaluation of disinfection of gutta percha comes using different chemical solutions after immersing into Enterococcus Faecalis
DOI:
https://doi.org/10.61841/65ygbx63Keywords:
Gutta Percha cones, sodium hypochlorite, cidex solution, 17% Edta, Endodontic Treatment, Root Canal therapyAbstract
Gutta percha cones are radiopaque cones that are used to fill and seal the canal after undergoing endodontic therapy.The success of endodontic treatment is determined by the biochemical preparation and the use of sterilized techniques during the procedure.Care should be taken during the treatment to avoid contamination of instruments and filling materials as this is to avoid cross - infection in the root canal .Various disinfection have been used to sterilize guttad-percha cones including sodium hypochlorite (NaOCl).The aim of this study is to evaluate and compare the efficiency of 3% of NaOCl , Cidex solution and 17% of Edta in disinfecting gutta-percha cones after immersing it into bacterial suspension of enterococcus faecalis.Twelve gutta percha cones of size 70 were used in this study were divided into various groups depending upon the type of solution 3% NaOCl solution,Cidex Solution, 17% EDTA solution with time of exposure to each solution (15 min) and type of microorganism (Enterococcus Faecalis )Sodium Hypochlorite was found to be effective disinfectant.The outcome of this study confirmed the efficacy of 3% NaOCl in the disinfection of gutta percha cones.
Downloads
References
[1] Mahesh Martur Chandrappa, Nofal Mundathodu, and Ashwija Shetty. Disinfection of gutta-percha cones using three reagents and their residual effects. J Conserv Dent. 2014 Nov–Dec; 17(6):571–574.
[2] Roberta Redmerski, Joice Renata Bulla, Tatiana Moreno, Lourdes Botelho Garcia, Celso Luiz Cardoso. Disinfection of gutta-percha cones with chlorhexidine. Braz J Microbiol. 2007; 38(4): São Paulo Oct./Dec.
[3] Pradeep K., K.H. Kidiyoor, Pavithra Jain, Nageshwar Rao. Chair side disinfection of gutta-percha points – An in vitro comparative study between 5 different agents at different concentrations.
[4] Linke H.A., Chohayeb A.A. Effective surface sterilization of gutta-percha points. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1983; 5:73–7.
[5] Gomes B.P., Vianna M.E., Matsumoto C.U., Rossi Vde P., Zaia A.A., Ferraz C.C., et al. Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005; 100:512–7.
[6] Retamozo B., Shabahang S., Johnson N., Aprecio R.M., Torabinejad M. Minimum contact time and concentration of sodium hypochlorite required to eliminate Enterococcus faecalis. J Endod. 2010; 36:520–3.
[7] Senia E.S., Marraro R.V., Mitchell J.L., Lewis A.G., Thomas L. Rapid sterilization of gutta-percha cones with 5.25% sodium hypochlorite. J Endod. 1975; 1:136–40.
[8] Prado M., de Assis D.F., Gomes B.P., Simão R.A. Effect of disinfectant solutions on the surface free energy and wettability of filling material. J Endod. 2011; 37:980–2.
[9] Lee M.S. An Experimental Study of the Effect of the Various Antiseptic Storage Solutions on Physical Properties of Gutta-Percha Cone. MS Thesis, Yonsei Dental College; 1989.
[10] Norhayati Luddin, Hany Mohamed Aly Ahme. The antibacterial activity of sodium hypochlorite and chlorhexidine against Enterococcus faecalis: A review on agar diffusion and direct contact methods. J Conserv Dent. 2013; 16(1):9–16. doi:10.4103/0972-0707.105291.
[11] Carlos Estrela, R.A. Estrela, Eduardo Luis Barbin, Júlio César E. Spanó, Melissa A. Marchesan, Jesus D. Pécor. Mechanism of Action of Sodium Hypochlorite. Braz Dent J. 2002; 13(2):113–117.
[12] Zahed Mohammadi, Sousan Shalavi, Hamid Jafarzadeh. Ethylenediaminetetraacetic acid in endodontics. Eur J Dent. 2013; 7(Suppl 1):S135–S142. doi:10.4103/1305-7456.119091.
[13] Nabeshima C.K., Machado M.E., Britto M.L., Pallotta R.C. Effectiveness of different chemical agents for disinfection of gutta-percha cones. Aust Endod J. 2011; 37:118–21.
[14] da Motta P.G., de Figueiredo C.B., Maltos S.M., Nicoli J.R., Ribeiro Sobrinho A.P., Maltos K.L., et al. Efficacy of chemical sterilization and storage conditions of gutta-percha cones. Int Endod J. 2001; 34:435–9.
[15] Kunigk L., Almeida M.C. Action of peracetic acid on Escherichia coli and Staphylococcus aureus in suspension or settled on stainless steel surfaces. Braz J Microbiol. 2001; 32:38–41.
[16] Salvia A.C., Teodoro G.R., Balducci I., Koga-Ito C.Y., Oliveira S.H. Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones. Braz Oral Res. 2011; 25:23–7.
[17] Tandjung L., Waltimo T., Hauser I., Heide P., Decker E.M., Weiger R. Octenidine in root canal and dentine disinfection ex vivo. Int Endod J. 2007; 40:845–51.
[18] Patters M.R., Anerud K., Trummel C.L., Kornman K.S., Nalbandian J., Robertson P.B. Inhibition of plaque formation in humans by octenidine mouthrinse. J Periodontal Res. 1983; 18:212–9.
[19] Frank R.J., Pelleu G.B. Jr. Glutaraldehyde decontamination of gutta-percha cones. J Endod. 1983; 9:368–71.
[20] Sahinkesen G., Oktay E.A., Er Ö., Koçak M.M., Kiliç A. Evaluation of residual antimicrobial effects and surface changes of gutta-percha disinfected with different solutions. J Contemp Dent Pract. 2011; 12:47–51.
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.