EFFECT OF PLANT EXTRACT IN SALMONELLA TYPHI ISOLATED FROM TYPHOID PATIENT
DOI:
https://doi.org/10.61841/rx3kzx46Keywords:
effect of plant extract inSalmonella typhi isolated from typhoid patientAbstract
Salmonella typhi is a member of S. serovar which cause "typhoid fever" , consider common health difficult in developing countries. The purpose of the research was to study plant extract of S. typhi in typhoid patient by molecular and bacteriological assay .A total of 90 blood samples aged between (10-60) years ,were involved in this study. blood samples were cultured directly on brain heart infusion broth .After that sub cultured of isolates on MacConkey agar and Hekton enteric agar and S.S agar to find the Salmonella typhi then identified by biochemical, molecular assay and antibiotic sensitive test. After that preparation of plant extracts from zingiber and acacia , fractionation with soxholet extractor and isolate the active material by HPLC , evaluation its potency on Salmonella typhi isolates by well diffusion and disk diffusion test. Results showed that there was 4 Salmonella typhi isolates from blood culture, as well as, Flic gene success in amplification of 450bp fragment for amino glycoside resistant , wherever Plant extracts may be show highest inhibition zone diameter of S. typhi reached by action of zingiber extract . while acacia extract was accompanied the lowest inhibition effect for S. typhi growth
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