THE EFFECT OF BEVACIZUMAB ON ALPHA SMOOTH MUSCLE ACTINEXPRESSION AND FIBROBLAST COUNT TO PREVENT POSTTRABECULECTOMY FIBROSIS IN NEW ZEALAND WHITE RABBIT

Authors

  • Sekar Ayu Sitoresmi Ophthamology Department, Medical Faculty of Airlangga University, Surabaya 60131, Indonesia Author
  • Nurwasis Ophthalmology Department, Medical Faculty of Airlangga University, Surabaya 60131, Indonesia. Author
  • Evelyn Komaratih Ophthamology Department, Medical Faculty of Airlangga University, Surabaya 60131, Indonesia Author
  • Heriyawatih Anatomical Pathology Department, Medical Faculty of Airlangga University, Surabaya 60131, Indonesia Author

DOI:

https://doi.org/10.61841/60tpr326

Keywords:

bevacizumab, fibrosis, glaucoma, post trabeculectomy

Abstract

Background: The wound healing process is the most common cause of glaucoma surgery failure that causes the incapability of controlling the intraocular pressure (IOP) and progressive optic nerve damage.

Objective: This study aimed to analyze the effect of Bevacizumab on myofibroblast and fibroblast in the trabeculectomy area of rabbit models in order to find a safer wound healing modulator to improve surgical outcomes.

Method: Sixteen New Zealand white rabbits aged 4-6 months and weighing between 2.5 and 3.5 kg underwent trabeculectomy on the right eye with a postoperative subconjunctival injection of BSS and Bevacizumab. Subjects were put into a control and bevacizumab groups. Examinations were done, and subjects were terminated and performed enucleation on postoperative day 14. Samples were histologically stained with hematoxyline-eosin to count the fibroblast.

Result: Mann Whitney u test and independent t-test were used to analyse the data. We found both less expression of alpha smooth muscle act and a lower fibroblast count in the bevacizumab group compared to the control group, which indicates less myofibroblast, fibroblast, and scarring potential in the trabeculectomy area. There is a significant decrease in expression of α-SMA in the bevacizumab group compared to the control group (p = 0.0195), and with an independent t-test, we found less fibroblast in the bevacizumab group significantly (p = 0.0005).

Conclusion: Bevacizumab inhibits fibroblast proliferation and its differentiation to myofibroblast, which leads to less collagen production and fibrosis. 

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References

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Published

30.04.2020

How to Cite

Ayu Sitoresmi, S., Nurwasis, Komaratih, E., & Heriyawatih. (2020). THE EFFECT OF BEVACIZUMAB ON ALPHA SMOOTH MUSCLE ACTINEXPRESSION AND FIBROBLAST COUNT TO PREVENT POSTTRABECULECTOMY FIBROSIS IN NEW ZEALAND WHITE RABBIT. International Journal of Psychosocial Rehabilitation, 24(2), 4043-4048. https://doi.org/10.61841/60tpr326