Effect of Some Environmental Stress Factors in Staphylococcus aureus' Biofilm
DOI:
https://doi.org/10.61841//IJPR/V24I10Keywords:
Staphylococcus aureus, biofilm, sodium nitrite, temperature, glucoseAbstract
The purpose of this research was to investigate if S.aureus work the formation of biofilms
and also to evaluate the impacts of environmental factors on the formation of biofilms. It
was shown that the pathogen can form biofilms, which have a negative impact on the
health of the organism, leading to resistance to environmental stress factors. This study
was designed by use Microtitre-plate(MP) technique for examination of biofilm formation
of S.aureus and their relationship with some environmental stress factors, which were
included temperature, glucose and sodium nitrate. The effects of biofilm formation were
tested with Luria-Bertani broth (LB) supplement varying concentrations of glucose (0.1;
0.5;1.0;2.0;3.0)%, sodium nitrite (0.05;0.1;0.15;0.2)%, as well were incubated the medium
at different temperatures (25;37;45)°C, individually. It was observed that the addition of
glucose (3.0%) was affected by the ability isolates to adhere to the MP-surface. Glucose
was showing a positive effect on the formation of isolates of the biofilm while sodium
nitrite was negatively affected. Where the isolates were produced the least amount of
biofilm at the concentration (0.2%) sodium nitrite. Interestingly, isolates were given the
high rate of formation of the biofilm at high temperature 45°C, which has resulted in their
classification among the moderate values in the composition of the biofilm.
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