THE EFFECT OF MITOMYCIN C ON POSTOPERATIVE PERITONEAL ADHESIONS IN MALE RATS
DOI:
https://doi.org/10.61841/ets82e19Keywords:
Mitomycin C, postoperative, peritoneal adhesions,, RatAbstract
Postoperative peritoneal adhesions contribute to high morbidity and mortality rates. Fibroblast proliferation is one of the risk factors. Mitomycin C (MMC) is known to inhibit fibroblast proliferation. We herein evaluate the effect of MMC on the peritoneal adhesion in the animal model of peritoneal irrigation.Sixty-four male Wistar rats underwent a midline incision laparotomy and then were randomly assigned to eight groups. The peritoneal cavity was rinsed with injectable normal saline (NS), MMC at doses of 0.5 or 1 mg/kg with or without NS, followed by an immediate suction. The peritoneal cavity in three groups was rinsed with NS or MMC at doses of 0.5 or 1 mg/kg for 3min before suction. After 6 weeks, a laparotomy was again performed on each rat andthe adhesions were scored macroscopically withCanbaz scoring system.Animals treated with immediate MMC 1 or 3 mg/kg had the lowest incidence of postoperative adhesion. The adhesions in groups receivedeach dose of MMC, with or without saline,were significantly reduced compared with the saline group. The adhesion score was significantly lower in MMC groups (0.5 and 1mg/kg) vs. each dose of MMCs +saline or each dose of MMCs for 3min. Notably, the adhesion score was not reduced with MMC 1mg/kg (3min) compared with NS (3min), while adhesions were significantly decreased with MMC 0.5mg/kg (3min). There was no difference between NS and NS (3min).Immediate peritoneal rinsing with MMCduring the laparotomy seems to be effective in preventing the formation of postoperative adhesions.
Downloads
References
1. Pucci MJ. Fundamentals of General Surgery: Springer; 2018.
2. Townsend Jr CM, Beauchamp RD, Evers BM, Mattox KL. Sabiston Textbook of Surgery International Edition: Elsevier Health Sciences; 2015.
3. ten Broek RP, Stommel MW, Strik C, van Laarhoven CJ, Keus F, van Goor H. Benefits and harms of adhesion barriers for abdominal surgery: a systematic review and meta-analysis. The Lancet. 2014;383(9911):48-59.
4. Oncel M, Kurt N, Remzi FH, Sensu SS, Vural S, Gezen CF, et al. The effectiveness of systemic antibiotics in preventing postoperative, intraabdominal adhesions in an animal model. Journal of Surgical Research. 2001;101(1):52-5.
5. Bothin C, Midtvedt T, Perbeck L. Orally delivered antibiotics which lower bacterial numbers decrease experimental intra-abdominal adhesions. Langenbeck's archives of surgery. 2003;388(2):112-5.
6. Moris D, Chakedis J, Rahnemai-Azar AA, Wilson A, Hennessy MM, Athanasiou A, et al. Postoperative abdominal adhesions: clinical significance and advances in prevention and management. Journal of Gastrointestinal Surgery. 2017;21(10):1713-22.
7. Çubukçu A, Alponat A, Gönüllü NN, Özkan S, Erçin C. An experimental study evaluating the effect of mitomycin C on the prevention of postoperative intraabdominal adhesions. Journal of Surgical Research. 2001;96(2):163-6.
8. Brunton LL, Chabner BA, Knollmann BC. Goodman & Gilman’s the pharmacological basis of therapeutics, 12e. Pharmacotherapy of the Epilepsies, Valproic Acid. 2011.
9. Cruz OA. Evaluation of mitomycin to limit postoperative adhesions in strabismus surgery. Journal of pediatric ophthalmology and strabismus. 1996;33(2):89-92.
10. Wilkins M. Wilkins M, Indar A, Wormald R. Intraoperative mitomycin C for glaucoma surgery. Cochrane Database Syst Rev. 2005(4).
11. Rahbar R, Jones DT, Nuss RC, Roberson DW, Kenna MA, McGill TJ, et al. The role of mitomycin in the prevention and treatment of scar formation in the pediatric aerodigestive tract: friend or foe? Archives of otolaryngology–head & neck surgery. 2002;128(4):401-6.
12. Soltani S, Foruzesh Fard M, Danaie N. The effect of mitomycin and dexamethasone on peritoneal adhesion formation after abdominal surgery in Rat. Koomesh. 2005;6(4):297-304.
13. Canbaz MA, Üstün C, Koçak I, Yanik FF. The comparison of gonadotropin-releasing hormone agonist therapy and intraperitoneal Ringer's lactate solution in prevention of postoperative adhesion formation in rat models. European Journal of Obstetrics & Gynecology and Reproductive Biology. 1999;82(2):219-22.
14. Duron JJ. Postoperative intraperitoneal adhesion pathophysiology. Colorectal disease. 2007;9:14-24.
15. Ivarsson M, Bergström M, Eriksson E, Risberg B, Holmdahl I. Tissue markers as predictors of postoperative adhesions. British journal of surgery. 1998;85(11):1549-54.
16. Rout UK, Diamond MP. Role of plasminogen activators during healing after uterine serosal lesioning in the rat. Fertility and sterility. 2003;79(1):138-45.
17. Sun Y, Ge Y, Fu Y, Yan L, Cai J, Shi K, et al. Mitomycin C induces fibroblasts apoptosis and reduces epidural fibrosis by regulating miR-200b and its targeting of RhoE. European journal of pharmacology. 2015;765:198-208.
18. Yanoff M. Ophthalmic Diagnosis & Treatment: JP Medical Ltd; 2014.
19. Lee H, Jeong H, Lee CM, Shin J-A, Jang S-W, Lee JH, et al. Antifibrotic Effects of Sakuraso-Saponin in Primary Cultured Pterygium Fibroblasts in Comparison With Mitomycin C. Investigative ophthalmology & visual science. 2019;60(14):4784-91.
20. Lindquist TP, Lee WB. Mitomycin C-associated scleral stromalysis after pterygium surgery. Cornea. 2015;34(4):398-401.
21. Adachi W, Koike S, Rafique M, Kajikawa S, Kaneko G, Kuroda T, et al. Preoperative intraperitoneal chemotherapy for gastric cancer, with special reference to delayed peritoneal complications. Surgery today. 1995;25(5):396-403.
22. Jansen M, Fass J, Langejürgen E, Forsch S, Tietze L, Schumpelick V. Effects of intraperitoneal mitomycin C adsorbed on activated carbon on adhesion formation and mesothelial cells in vitro. European Journal of Surgery. 2000;166(7):572-6.
23. Kapoor d., lal b., gupta p. (2016) a malleable technique for future coating process–solventless coating. Journal of Critical Reviews, 3 (2), 55-59.
24. Tarlaci, S.The measurement problem in quantum mechanics: Well, where's the problem?(2012) NeuroQuantology, 10 (2), pp. 216-229.
25. Rinkus, G.J.Quantum computation via sparse distributed representation (2012) NeuroQuantology, 10 (2), pp.
311-315.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
