Effect of CNP additive to diluents during capacitation on live percentage and pH of bull epididymal spermatozoa.
Abattoir male gonads specimens were collected from Al-Shoáalla abattoir from June 2019 until November 2019 in an average of 2 visits per week. This study was designed to evaluate the optimum effect of C-type natriuretic peptide on the caudal epididymal spermatozoa characteristics from a healthy slaughter bull. Dissected and separated the epididymis from the entire testicle made by a small scissor. The caudae were injected with 5-8 ml of the TCM199 media containing 100 IU/ml penicillin-streptomycin and 100 IU/ml Nystatin using an 18 gauge needle attached toa 5-ml syringe then aspirated. After collection of Spermatozoa samples of cauda epididymis were evaluated under the light microscope has individual motility less than 70% of were rejected. The caudal epididymal Spermatozoa samples transported from petri dish to a 10 ml test tube containing 2 ml maturation media (TCM199) that contain 100 IU/ml penicillin-streptomycin and 100 IU/ml Nystatin; the test tube incubated in a 5% CO2 incubator at 39 Cº for 4 hours for sperm maturation, the presence of distal protoplasmic droplet was the criteria of sperm maturation. Then the samples divided into three equal groups control one, low concentration 10-7 mol/L, and high concentration 10- 6 mol/L. It evaluated the effect of C-type natriuretic peptide (CNP) during capacitation on the live percentage and pH of bull epididymal spermatozoa. The live percentage and pH of sperm were analyzed at 5 min, 10 min, 15 min, 30 min, 45 min, 60 min, 120 min, and 180 min for each group. A total of 20 caudal epididymides were studied during the period of the experiment. Evaluation of spermatozoa parameters as a stained smear for dead and alive spermatozoa was conducted in addition to pH; all results were recorded.
