Vascular Invasion in Hepatocellular Carcinoma and Matrix Metalloproteinase-14 Gene Polymorphisms
Mostafa Tharwat Mostafa Mohamed, Soha Esmat Ahmed Khoushed, Nermin Raafat Abdel Fattah, Ahmed MohamedEL Gebaly Ahmed
Background: Matrix metalloproteinase (MMP) 14 is a cell surface proteinase that considered a key protein of activity against extracellular matrix components and associated with tumor invasion, metastasis and angiogenesis. MMP14 is overexpressed in HCC, expression of MMP14 is correlated with advanced tumor stageas MMP14 gene is overexpressed in highly invasive hepatocellular carcinoma.The purpose of this study was to examine whether the MMP14 gene polymorphisms are associated with Vascular invasion.
Methods:A case control study was carried in Tropical Medicine Department in Zagazig University Hospitals and Medical Biochemistry Department Faculty of Medicine in Zagazig University, The study included 540 subject, classified into 3 groups HCC, Cirrhotic, control (180 in each group)MMP-14 gene polymorphism analysis in the promoter of the MMP-14 gene(+7096) was detected by polymerase chain reaction-restriction fragment length polymorphism
Results: It was found that the most frequent age category affected by HCC was 51–60 years, 83.3% were males, 88.9% of HCC were HCV,Vascular invasion was found in 44.4% of HCC,it was found that 86.2% of HCC patients with vascular invasion had TC genotype.
Conclusion:Our study results demonstrated that TC polymorphism of MMP 14 (+7096) is associated with increased risk of vascular invasionin HCC