Role of Matrix Metalloproteinase-14 Gene Polymorphisms in Portal Vein Thrombosis Development in Patients with Hepatocellular Carcinoma
MostafaTharwatMostafaMohamed, SohaEsmat Ahmed Khoushed, NerminRaafat AbdelFattah, Ahmed Mohamed EL Gebaly Ahmed
Background: Matrix metalloproteinase (MMP) 14 is a cell surface proteinase that considered a key protein of activity against extracellular matrix components and promotes the invasion/metastasis of cells. MMP14 is overexpressed in HCC, expression of MMP14 is correlated with advanced tumor stageand can be considered as poor prognostic factor.The purpose of this study was to examine whether the MMP14 gene polymorphisms are associated with the susceptibility to PVT.
Methods:A case control study was carried in Tropical Medicine Department in Zagazig University Hospitals and Medical Biochemistry Department Faculty of Medicine in Zagazig University, The study included 540 person, classified into 3 groups HCC, Cirrhotic, healthy (180 in each group)MMP-14 gene polymorphism analysis in the promoter of the MMP-14 gene(+7096) was detected by polymerase chain reaction-restriction fragment length polymorphism
Results: It was found that the most frequent age category affected by HCC was 51–60 years, 83.3% were males, 88.9% of HCC were HCV,PVT was found in 44.4% of HCC, TC genotype was found to be significantly higher among HCC patients when compared to the control group (77.8% versus 16.7% respectively), it was found that 86.2% of HCC patients with PVT had TC genotype.
Conclusion: Our study results demonstrated that single nucleotide polymorphism of MMP 14 (+7096) among the Egyptian population is associated with increased risk of PVT in HCC