Changes of Enzymes in Epididymal Fluid during Different Steps of Processing (After Dilution, After Cooling and Post Thawing) of Buffalo Bulls

1Asmaa S. Alilawy, SouhaylaO. Hussain

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Abstract:

Epididymal fluid (GOT,GPT, and LDH) were assessed in diluted (Tris-egg yolk-citric acid extender 1:5) and Thawing of 0.5 straws was done at 37 °C for 30 seconds using a container contains water at 37°Cepididymal fluid of buffalo bull (control group). Diluted, cooled (4°C/ 2-4 hours) and equilibrated (at 4°C for 2 hrs) epididymal fluid straws were frozen.These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of enzyme was studied by assessing GOT inepididymal fluidof thawed semen indicate that lowest significant (P>0.05) values in T4 (30.64 ± 0.45 mM)and highest significant (P>0.05) value was in T1(57.17 ±0.53mM). While,GPT at post thawing waslowest significant (P> 0.05) Values in T4(0.59 ±0.009 mM), and highest significant (P> 0.05) values in T1 (0.74 ±0.006mM ) in comparison with others T2 and T3 (0.70±0.00and 0.65 ±0.008mM).However, values of enzyme (LDH) at post thawing values indicate was lowest significant (P>0.05) values in T4(1433.66±99.512 mM), and highest significant (P> 0.05) values in T1 (3802.50±146.17 mM) in comparison with others T2(2486.92±111.80mM) and T3 (1892.16 ± 113.311 mM) increased in GOT,GPT and LDH activity during different steps of freezing, with highest significant was in post thawing and lowest significant in after dilution.

Keywords:

Epididymal Sperm, Buffalo bull, L-Carnitine, Acrosome integrity

Paper Details
Month11
Year2020
Volume24
IssueIssue 10
Pages5683-5692