Spectrophotometric Determination of Clonazepam Drug in Pure form and Pharmaceutical Preparation by Diazotization Reaction
1Hajir S. Alteemi and Kassim H. Kadim
By diazotization coupling reaction as accurate, selective and rapid spectrophotometric method for determination drug of clonazepam by reliant on azo-coupling reaction between CZP and 1,2-phenylenediamine for To achieve the purpose of obtaining the colored product with a maximum absorbance 480nm. For the conditions reaction have been studied and optimized. The linearity range for clonazepam was (0.1_6μg\ml) ,while the detections limit 0.04327and quantification limit 0.14425μg\ml The Molar Absorptivity 8.505×104 L/mol.cm with Sandal‘s sensitivity 0.00370 μg\cm2. Eventually this methods were successfully completed to determination of CZP in pure from and commercial form
Spectrophotometric Determination, Clonazepam, Azo-coupling Reaction