The Role of Caspases in Inflammatory Responses against Gram Negative Infections

Pseudomonas aeruginosa is emerged as one of the utmost difficult nosocomial pathogens. The present study aims to estimate the role of caspases in inflammatory responses against Pseudomonas aeruginosa infection. This study was performed on 50 isolates infected patients. the diagnosis isolates depending on the macroscopic, microscopic,biochemical and API20E tests, results show that the percentage of isolates according to clinical sources were (38%) from burn, (24%) from urine,(6%) from ear swabs,(20%) from sputum and (12%) from wounds. A different abilities to resist antibiotics included Amoxicillin- clavulanic acid, Trimethoprimsulfamethoxazole and Erythromycins how effectiveness against P.aeruginosa. The ability of biofilm formation as a virulence factor by Micro-titer plate method (MTP), results revealed that (92%) of P. aeruginosa have ability to form biofilm. Molecular diagnosis of isolates implemented by 16SrDNA gene in addition to detect the virulence gene (Flic gene), the results showed (90%) of isolates were positive. the results of the casp-1 patients sequencing showed 70% have heterozygous nucleotide and the level of IL-1β showed no significant differences between infected and non-infected individuals. Comparison between IL-1β level with patients age group where the F, G and H group revealed a significant differences. The comparison between sources infection recorded higher significant differences between cystic fibrosis compared with wound and UTI source.