Molecular Detection of IntegronclassI Gene in Pseudomonas Aerginosa Isolated from Otitis Media

The study aimed to investigating the Pseudomonas aerginosa from patients suffering otitis media infection. The study included of the bacterial diagnosis based on relied diagnostic procedures. A total of 40 specimens collected from (ear swab) in Al-Najaf province within period from September to December 2019.These patients ranged from 30-75 years. The male more than female. Primary identification was depended on Gram stain and biochemical tests. Finally identification with vitek 2 system was done, the results demonstrate the 30( 75%) to Pseudomonas aeruginosa. Genetically, study for gene encoding integrin of P. aeruginosa by using PCR technique. The results indicated, all P. aeruginosa isolate contain IntClassIgene that PCR amplification revealed that 30(75%) of P. aeruginosa carried int1gene.