Effects of Aspergillus spp. Phospholipase to Induce Histological and Biochemical Changes in Male Rats

This study was achieved to exhibit effects of Aspergillus spp. phospholipase on rats (in vivo). In this study, 18 adult albino male rats were used which divided to three groups including the control group (received ad libidium), second group (injected with 0.05 ml/per animal of phospholaipase) and the third group (injected with 0.1 ml/per animal of phospholaipase) for 3 weeks. The results showed high significant increase (p<0.05) in levels of IL- 1α, IL-2, IL-6 and IL-8 in second and third groups compared with the control group. Oxidative stress factor in second and third groups exhibited a significant increase (p<0.05) in levels of MDA (malonedialdehyied) and glutathione (GSH) compared with the control group. About the histological changes, the liver sections that prepared from the second and third groups showed the degeneration of hepatocytes, thickening wall of central veins with inflammatory cells infiltration and different changes in nuclei in many hepatocytes. It was concluded that phospholipase that extracted from Aspergillus spp. led to remarkable histological and biochemical changes in rats.